Master thesis project in Molecular biology/cell/RNA biology lab

Interested students are welcome to join the RNA biology group at CGC (Chemical Genomics Center) of the Max-Planck Society in Dortmund under the supervision of Dr. Jochen Imig to carry out a research practical or master thesis.

At the Imig group we are interested on discovering long non-coding RNA/miRNA networks controlling cancer metastasis. We use different methods to study these networks such as CRISPR-Cas9 screening and RNA immunoprecipitation (in particular miR-CLIP). We aim to optimize a novel dual Cas9 system to screen for simultaneous knock-out of two RNA molecules in a cancer cell; this will give us information about RNA molecules that have direct genetic interactions and cooperate together in a cell. We also aim to define miRNA-lncRNA interactome in cancer cells by advancing a known RNA immunoprecipitation method called miR-CLIP.  

 At CGC we work closely with four pharmaceutical companies (Merck, AstraZeneca, Pfizer and Roche) that sponsor our projects. 

 Applicant qualifications:

  • Bachelor in biology, biochemistry or similar field
  • Student of master degree in a relevant field (background in molecular biology)
  • Good knowledge of English
  • Passionate about science, ambitious and hard-working student
  • Good theoretical knowledge in cell and molecular biology
  • Familiar with concepts of molecular biology techniques
  • Previous hands on experience in molecular/cell biology techniques would be desirable but not mandatory (e.g. PCR, bacterial cloning, SDS-page, cell culture, etc.)

 Projects will generally include following methods:

  • PCR and Real time PCR
  • DNA cloning
  • Cell culture techniques
  • Protein-RNA interaction (immunoprecipitation)

 Master thesis will be based on one of the following subjects:

  • Project1: Optimization of a specific immunoprecipitation technique with biochemical approaches,
  • Project2: Optimization of a CRISPR-Cas9 tool by molecular cloning

 What you will gain:

If you choose project 1) you will learn about non-coding RNAs and their network. You will gain expertise in biochemical method development. You will learn how to culture and transfect cells. 

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