Publications

Selected publications:

"Chimeric deubiquitinase engineering reveals structural basis for specific inhibition of USP30 and a framework for DUB ligandability“

Kazi NH, Klink N, Gallant K, Kipka GM, Gersch M,

bioRxiv 2024. doi.

"Monovalent Pseudo-Natural Product Degraders Supercharge the Native Degradation of IDO1 by KLHDC3“

Hennes E, Lucas B, Scholes NS, Cheng XF, Scott DC, Bischoff M, Reich K, Gasper R, Lucas M, Xu TT, Pulvermacher LM, Dötsch L, Imrichova H, Brause A, Naredla KR, Sievers S, Kumar K, Janning P, Gersch M, Murray PJ, Schulman BA, Winter GE, Ziegler S, Waldmann H,

bioRxiv 2024. doi.

“Discovery and mechanism of K63-linkage-directed deubiquitinase activity in USP53”

Wendrich K, Gallant K, Recknagel S, Petroulia S, Kazi NH, Hane, JA, Führer S, Bezstarosti K, O’Dea R,

Demmers J, Gersch M,

bioRxiv 2024. doi.

Nat Chem Biol 2024, accepted in principle.

“Variety in the USP deubiquitinase catalytic mechanism”

Keijzer N, Priyanka A, Stijf-Bultsma Y, Fish A, Gersch M, Sixma TK,

Life Sci Alliance 2024, 7(4), e202302533. doi.

"N-Cyanopiperazines as Specific Covalent Inhibitors of the Deubiquitinating Enzyme UCHL1"

Schmidt M, Grethe C, Recknagel S, Kipka GM, Klink N, Gersch M,
Angew. Chem., Int. Ed., 2024, 63(12), e202318849. doi

"Native semi-synthesis of isopeptide-linked substrates for specificity analysis of deubiquitinases and Ubl proteases“

Zhao Z, O'Dea R, Wendrich K, Kazi N, Gersch M,

J Am Chem Soc, 2023, 145(38), 20801–20812. doi.

"Molecular basis for Ubiquitin/Fubi cross-reactivity in USP16 and USP36"
O’Dea R, Kazi N, Hoffmann-Benito A, Zhao Z, Recknagel S, Wendrich K, Janning P, Gersch M,
Nat Chem Biol, 2023, 19(11), 1394–1405. doi.

"Structural basis for specific inhibition of the deubiquitinase UCHL1”
Grethe C, Schmidt M, Kipka GM, O'Dea R, Gallant K, Janning P, Gersch M,
Nat Commun, 2022, 13(1), 5950. doi

“Distinct USP25 and USP28 Oligomerization States Regulate Deubiquitinating Activity”
Gersch M, Wagstaff JL, Toms AV, Graves B, Freund SMV, Komander D,
Mol Cell, 2019, 74, 436–451.

“Mechanism and regulation of the Lys6-selective deubiquitinase USP30”
Gersch M, Gladkova C, Schubert A, Michel M, Maslen S, Komander D,
Nat Struct Mol Biol, 2017, 24(11), 920–930. (Also highlighted in Nat Chem Biol).

“Barrel-shaped ClpP proteases display attenuated cleavage specificities”
Gersch M, Stahl M, Poreba M, Dahmen M, Dziedzic A, Drag M, Sieber SA
ACS Chem Biol, 2016, 19(11), 389–399.

“AAA+ chaperones and acyldepsipeptides activate the ClpP protease via conformational control”
Gersch M, Famulla K, Dahmen M, Goebl C, Malik I, Richter K, Korotkov VS, Sass P, Ruebsamen-Schaeff H, Madl T, Broetz-Oesterhelt H, Sieber SA,
Nat Commun, 2015, 19(6), 6320.

“Intact protein mass spectrometry of 20S proteasomes reveals complex integrity, phosphorylation stoichiometry and inhibitor specificity”
Gersch M, Hackl M, Dubiella C, Dobrinevski A, Groll M, Sieber SA,
Chem Biol, 2015, 22(3), 404–411.

"Disruption of oligomerization and dehydroalanine formation as mechanisms for ClpP protease inhibition"
Gersch M, Kolb R, Alte F, Groll M, Sieber SA,
J Am Chem Soc, 2014, 136(4),1360–1366.

"The Mechanism of ClpP Inhibition",
Gersch M, Gut F, Korotkov V, Lehmann J, Böttcher T, Rusch M, Hedberg C, Waldmann H, Klebe G, Sieber SA,
Angew Chem Int Ed, 2013, 52(10), 3009–3014.

"Electrophilic natural products and their biological targets",
Gersch M, Kreuzer J, Sieber SA,
Nat Prod Rep, 2012, 29, 659–682. (Review)

"Insights into the structural network responsible for oligomerization and activity of the bacterial virulence regulator caseinolytic protease P (ClpP)"
Gersch M, List A, Groll M, Sieber SA,
J Biol Chem, 2012, 287(12), 9484–9494.